Mouse brains expressed reporter genes which had been introduced by in vivo electroporation: upper panels, E15.5 telencephalons expressing EYFP, 2days after electroporation at E13.5; lower panels, P15 telencephalons expressing DsRed (left) and EYFP (right), after electroporation at E13.5 (left) and E16.5 (right). Scale bars, 200 µm.
Published in "Efficient Gene Transfer into the Embryonic Mouse Brain Using in vivo Electroporation." Developmental Biology 240, 237-246(2001).
Detailed protocols have also been published:
"In vivo electroporation in the embryonic mouse central nervous system." Nature Protocols 1, 1552-1558 (2006).
"Embryonic in vivo electroporation in the mouse." Methods in Enzymology 477 "Guide to Techniques in Mouse Development, Part B" pp 37-50 (2010).
