[updates]

Overview

 Upon technical innovations of big-data as well as omix analysis, the cutting-edge technologies such as single-cell sequencing and genome-editing system is emerging a big paradigm shift in the field of molecular biology and diagnosis. This isbonafide a kind of molecular biological movement of the Renaissance to answer the question of 120 years ago “D'où Venons Nous Que Sommes Nous Où Allons Nous”, a testament of Paul Gauguin. These technologies enable us, not only to dynamically,qualitatively, visually and systematically understand underling mechanisms of single gene, single molecule, single cell and their complicated networks in vivo, also to pursue the mechanistic insight into “Life, Aging, Disease and Death”, andthereby control them. Our lab organizes research projects, focusing on tumor & stem cell biology, regenerative medicine, aging as well as disease pathophysiology with the theme in molecular biology and diagnosis of “Life-Aging-Disease-Death”,based on novel technologies & methodologies.

Department of Molecular Diagnosis
Professor Tomoaki Tanaka, M.D., Ph. D.




Research & Education

  1. Application of omix analyses including genomics, proteomics, metabolomics and transcriptomics to elucidate the underlying mechanisms, particularly focusing on the regulatory system of transcriptional network and epigenetics for a variety of human disorders such as cancer, aging, immune disorders and life-style related disease.
  2. Elucidation of role of tumor suppressor pathways and oncogenic signals as well as non-coding RNAs in the regulation of stem cell functions, nuclear reprogramming process as well as stem cell commitment & differentiation, especially for endocrine organs.
  3. Application of genome-editing system using CRISPR/Cas9 in vitro & vivo, to reveal and understand the molecular pathogenesis of cancer, endocrine disorders, aging-associated & life-style-related disease including NASH (non-alcoholic steatohepatitis) and obesity.
  4. Technical innovations of genetic testing & clinical sequencing as well as genetic counseling for next generation of personalized medicine.

Recent Publications

    2019
  1. Nagano H, Kono T, Saiga A, Kubota Y, Fujimoto M, Saulo J.A. Felizola, Ishiwata K, Tamura A, Higuchi S, Sakuma I, Suzuki S, Koide H, Takeshita N, Sakamoto S, Yokote K, Nakamura Y, Ichikawa T, Uno T, Tanaka T. Aldosterone reduction rate after saline infusion test may be a novel prediction in patients with primary aldosteronism. J Clin Endocrinol Metab. (in press)
  2. Hosokawa H, Romero-Wolf M, Yang Q, Motomura Y, Levanon D, Groner Y, Moro K, Tanaka T, Rothenberg V. E. Cell-type specific actions of Bcl11b in early T-lineage and group 2 innate lymphoid cells. J Exp Med. (in press)
  3. Yoshida T, Nakayama A, Tamura A, Higuchi S, Sakuma I, Nagano H, Saulo J.A. Felizola, Takemoto M, Tatsuno I, Koide H, Yokote K, and Tanaka T. A case of Hashimoto’s thyroiditis with multiple drug resistance and high expression of efflux transporters. J Clin Endocrinol Metab. (in press)
  4. Takekoshi K, Satoh F, Tanabe A, Okamoto T, Ichihara A, Tsuiki M, Katabami T, Nomura M, Tanaka T, Matsuda T, Imai T, Yamada M, Asada S, Kawata N, Naruse M.. Correlation between urinary fractionated metanephrines in 24-hour and spot urine samples for evaluating the therapeutic effect of metyrosine: a subanalysis of a multicenter, open-label phase I/II study. Endocrine Journal. (in press)
  5. Sasahara Y, Kubota Y, Kosaka K, Adachi N, Yamaji Y, Nagano H, Akita S, Kuroda M, Tanaka T, Bujo H, Mitsukawa N. Adipose-Derived Stem Cells (ASCs) and Ceiling Culture-Derived Preadipocytes (ccdPAs) Cultured from Subcutaneous Fat Tissue Differed in their Epigenetic Characteristics and in Osteogenic Potential. Plast Reconstr Surg. 144(3):644-655 (2019) DOI:10.1097/PRS.0000000000005913
  6. Fengkai L, Kitajima S, Kohno S, Yoshida A, Tange S, Sasaki S, Okada O, Nishimoto Y, Muranaka Y, Nagatani N, Suzuki M, Masuda S, Tran C, Nishiuchi T, Tanaka T, Barbie D, Mukaida N, and Takahash C. RB inactivation induces a protumoral microenvironment via enhanced CCL2 secretion. Cancer Research, 79(15):3903-3915 (2019).
  7. Hashimoto N, Nagano H, Tanaka, T. The Role of Tumor Suppressor p53 in Metabolism and Energy Regulation, and Its Implication in Cancer and Lifestyle-related Diseases. Endocrine Journal., 33(6):485-496, (2019)
  8. Sakuma I, Nagano H, Yoshino I, Yokote K, Tanaka T. Ceritinib Aggravates Glycemic Control in Insulin-treated Patients with Diabetes and Metastatic ALK-positive Lung Cancer. Intern Med., 58(6). (2019). doi:10.2169/internalmedicine.1870-18.
  9. Tanaka S, Ishihara N, Suzuki S, Watanabe Y, Nagayama D, Yamaguchi T, Ohira M, Saiki A, Tanaka T, and Tatsuno I. Fatty acid desaturase 2 is up-regulated by the treatment with statin through geranylgeranyl pyrophosphate-dependent Rho kinase pathway in HepG2 cells. Sci Rep. 9(1);100009 (2019). https://doi.org/10.1038/s41598-019-46461-9.
    2018
  1. Hosokawa H, Romero-Wolf M, Yui A M, Ungerbäck J, Quiloan M, Matsumoto M, Nakayama I. K, Tanaka T. Rothenberg V. E. Bcl11b defines pro-T identity by site-specific cofactor recruitment and by repressing Id2 and Zbtb16. Nature Immunology. 19(12):1427-144, (2018)
  2. Nagano H, Hashimoto N, Nakayama A, Suzuki S, Miyabayashi U, Yamato A, Higuchi S, Fujimoto M, Sakuma I, Beppu M, Yokoyama M, Suzuki Y, Sugano S, Ikeda K, Tatsuno I, Manabe I, Yokote K, Inoue S, Tanaka T*. p53-Inducibe DPYSL4 Associates with Mitochondrial Supercomplexes and Regulates Energy Metabolism in Adipocytes and Cancer Cells. Proc. Natl. Acad. Sci. USA., 115 (33) 8370-8375, (2018)
  3. Hosokawa H, Ungerbäck J, Wang X, Matsumoto M, Nakayama I. K, Cohen M. S, Tanaka T. Rothenberg V. E. Transcription factor PU.1 represses and activates gene expression in early T cells by redirecting partner transcription factor binding. Immunity, 48(6):1119-1134. (2018)
  4. Tamura A, Ogasawara T, Fujii Y, Kaneko H, Nakayama A, Higuchi S, Hashimoto N, Miyabayashi Y, Komai E, Kohno T, Sakuma I, Suzuki S, Mayama T, Koide H, Yokote K, Ogawa Seishi, and Tanaka T*. Glucagonoma with necrolytic migratory erythema: metabolic profile and detection of biallelic inactivation of DAXX gene. J Clin Endocrinol Metab., 103(7):1-7. (2018)
  5. Sakuma I, Koide H, Yoshida T, Yamato A, Fujimoto M, Tamura A, Komai E, Kono T, Nagano H, Horiguchi K, Yokote K, and Tanaka T. Congestive heart failure secondary to a TSH-secreting pituitary adenoma aggravated by takotsubo cardiomyopathy in elderly patient. Endocrine Practice,, 4(2) e115-e123. (2018)
  6. Koide H, Shiga A, Komai E Yamato A, Fujimoto M, Tamura A, Kono T, Nakayama A, Takiguchi T, Higuchi S, Sakuma I, Nagano H, Hashimoto N, Suzuki S, Takeda Y, Shibuya M, Nishioka H, Yamada S, Inoshita N, Ishiwatari N, Horiguchi, Yokote K, Tanaka T. Prednisolone-responsive Postpertum IgG4-related Hypophysitis. Internal Medicine., 57(3) 367-375. (2018)
  7. Takayama KI, Suzuki T, Tanaka T, Fujimura T, Takahashi S, Urano T, Ikeda K, Inoue S. TRIM25 enhances cell growth and cell survival by modulating p53 signals via interaction with G3BP2 in prostate cancer. Oncogene, 37(16):2165-2180. (2018)
  8. Hosokawa H, Romero-Wolf M, Yui A M, Ungerbäck J, Quiloan M, Matsumoto M, Nakayama I. K, Tanaka T. Rothenberg V. E. Bcl11b defines pro-T identity by site-specific cofactor recruitment and by repressing Id2 and Zbtb16. Nature Immunology. 19(12):1427-144, (2018, Dec.) doi: 10.1038/s41590-018-0238-4
  9. Nagano H, Hashimoto N, Nakayama A, Suzuki S, Miyabayashi U, Yamato A, Higuchi S, Fujimoto M, Sakuma I, Beppu M, Yokoyama M, Suzuki Y, Sugano S, Ikeda K, Tatsuno I, Manabe I, Yokote K, Inoue S, Tanaka T*. p53-Inducibe DPYSL4 Associates with Mitochondrial Supercomplexes and Regulates Energy Metabolism in Adipocytes and Cancer Cells. Proc. Natl. Acad. Sci. USA., 115 (33) 8370-8375, (2018, Aug.) http://doi.org/10.1073/pnas.1804243115.
  10. Hosokawa H, Ungerbäck J, Wang X, Matsumoto M, Nakayama I. K, Cohen M. S, Tanaka T. Rothenberg V. E. Transcription factor PU.1 represses and activates gene expression in early T cells by redirecting partner transcription factor binding. Immunity, 48(6):1119-1134. (2018) doi:10.1016/j.immuni.2018.04.024
  11. Tamura A, Ogasawara T, Fujii Y, Kaneko H, Nakayama A, Higuchi S, Hashimoto N, Miyabayashi Y, Komai E, Kohno T, Sakuma I, Suzuki S, Mayama T, Koide H, Yokote K, Ogawa Seishi, and Tanaka T*. Glucagonoma with necrolytic migratory erythema: metabolic profile and detection of biallelic inactivation of DAXX gene. J Clin Endocrinol Metab., 103(7):2417-2423. (2018) doi:0.1210/jc.2017-02646
  12. Sakuma I, Koide H, Yoshida T, Yamato A, Fujimoto M, Tamura A, Komai E, Kono T, Nagano H, Horiguchi K, Yokote K, and Tanaka T. Congestive heart failure secondary to a TSH-secreting pituitary adenoma aggravated by takotsubo cardiomyopathy in elderly patient. Endocrine Practice,, 4(2) e115-e123. (2018) doi:org/10.4158/EP171968.CR
  13. Koide H, Shiga A, Komai E Yamato A, Fujimoto M, Tamura A, Kono T, Nakayama A, Takiguchi T, Higuchi S, Sakuma I, Nagano H, Hashimoto N, Suzuki S, Takeda Y, Shibuya M, Nishioka H, Yamada S, Inoshita N, Ishiwatari N, Horiguchi, Yokote K, Tanaka T. Prednisolone-responsive Postpertum IgG4-related Hypophysitis. Internal Medicine., 57(3) 367-375. (2018) doi:10.2169/internalmedicine.8446-16
  14. Takayama KI, Suzuki T, Tanaka T, Fujimura T, Takahashi S, Urano T, Ikeda K, Inoue S. TRIM25 enhances cell growth and cell survival by modulating p53 signals via interaction with G3BP2 in prostate cancer. Oncogene, 37(16):2165-2180. (2018) doi: 10.1038/s41388-017-0095-x





-- under construction --